報告書番号

U89030

タイトル（和文）

バクテリオファージによるスライム防除２．ファージの単離と溶菌効果の基礎的検討

タイトル（英文）

CONTROL OF MICROBIOFOULING USING BACTERIOPHAGE:2. DETECTION OF PHAGES AND FUNDAMENTAL STUDY OF THEIR LYTIC EFFECT ON FOULING BACTERIA

概要 （図表や脚注は「報告書全文」に掲載しております）

予め分離した付着細菌３０株を用いて，それらを溶菌するファージを昭和６２年１０月から６３年の１２月までほぼ月１回の間隔で海水より探索した。１７株の細菌が溶菌され，１８株のファージが単離できた。ファージ感受性細菌はいずれもグラム陰性の桿菌であるＰｓｅｕｄｏｍｏｎａｓ（１２株）Ａｃｉｎｅｔｏｂａｃｔｅｒ－Ａｃｈｒｏｍｏｂａｃｔｅｒ（３株），Ｖｉｂｒｉｏ（１株）およびＦｌａｖｏｂａｃｔｅｒｉｕｍ（１株）であった。ファージの出現頻度は細菌によって異なり，全く検出されなかったものからほぼ一年中検出されたものまで幅がみられた。増殖速度の速い細菌に対するファージの出現頻度は高く，一方，増殖速度の遅い細菌に対するそれは低い傾向が認められた。宿主域の明らかな宿主細菌ーファージ系において，ファージのスライム構成細菌増殖抑制効果が認められた。作用されるファージ数は，宿主細菌の０．１倍以上であれば顕著な

概要 （英文）

THE MICROBIOFOULING IN CONDENSER TUBES OF THERMAL POWER PLANTS MARKEDLY REDUCES COOLING EFFICIENCY. RECENTLY A METHOD FOR CONTROLING MICROBIOFOULING USING BACTERIOPHAGES HAS BEEN RECEIVING CONSIDERATION. IN THIS PAPER,STUDIES OF THE ECOLOGICAL RELATIONSHIP BETWEEN FOULING BACTERIA AND PHAGES,AND THE LYTIC ACTIVITY OF PHAGES TOFOULING BACTERIA ARE REPORTED. WITH THIRTY CULTURES OF THE ISOLATED FOULING BACTERIA FROM MICROBIAL FILM ON GLASS SUBSTRATUM IMMERSED IN FLOWING SEAWATER AT SENDAI BAY IN NORTH JAPAN,WE CARRIED OUT THE DETECTION AND ISOLATION OF PHAGES LYSING THE BACTERIA FROM SEAWATER FROM OCTOBER 1987 TO DECEMBER 1988 ABOUT ONCE PER MONTH. THE RESULTS OBTAINED WERE AS FOLLOWS :SEVENTEEN CULTURES WERE SENSITIVE TO PHAGES AND WERE FOUND TO BELONG TO PSEUDOMONAS(12 STRAINS),ACINETOBACTER ACHROMOBACTOR (3 STRAINS),VIBRIO (1 STRAIN) AND FLAVOBACTERIUM(1 STRAIN). EIGHTEEN STRAINS OF PHAGES WERE ISOLATED. A HIGH FREQUENCY OF PHAGE INCIDENCE WAS OBSERVED WITH THE CULTURES OF RAPID GROWING BACTERIA. BUT,FOR CULTURES OF SLOWGROWING BACTERIA,PHAGES WERE RARELY ISOLATED. UNDER DIFFERENT M.O.I(MULTIPLICITY OF INFECTION),THE LYTIC ACTIVITY OF PHAGE TO HOST BACTERIA WAS STUDIED. AT M. O.I. HIGHER THAN 0.1,THE PHAGE COULD EFFECTIVELY LYSEHOST BACTERIA AFTER 5-7 HR OF INFECTION AT M.O.I. LOWER THAN 0.01,THE LOW LEVEL OF BACTERIA CONCENTRATION WAS MAINTAINED WITH ONLY SLIGHT INCREASE UNTIL 48 HRS AFTER INFECTION. VARIOUS FILAMENTOUS BACTERIA WERE COMMONLY FOUND AS PREDOMINANT SPECIES IN CONDENSER TUBE MICROBIOFOULING COMMUNITIES. HOWEVER WE WERE UNABLE TO GROW THE FILAMENTOUS BACTERIA INTHE ESTABLISHED CULTURE MEDIUM USED IN THESE EXPERIMENTS. DEVELOPMENT OF CULTURE METHOD FOR THERE IS NECESSARY TO DEVELOP CONTROL METHODS OF FILAMENTOUS BACTERIA.

報告者

キーワード