報告書番号

V11021

タイトル（和文）

微細藻類によるバイオ燃料生産のための基礎的検討（その２）－炭化水素生産藻Botryococcus braunii Showa株の増殖モデルの構築－

タイトル（英文）

Growth model of hydrocarbon producing microalgae Botryococcus braunii strain Showa

概要 （図表や脚注は「報告書全文」に掲載しております）

変動する光量・温度環境下でのBotryococcus braunii Showa株の増殖を再現できるモデルを構築した。パラメータは、光量、温度、培養期間、およびin vivo蛍光値と現存量の関係式を用いた回帰により求めた。モデルの検証のため、Showa株を変動する光量・温度条件で培養してin vivo蛍光値と現存量の経時変化を調べた。実験の光量・温度データを入力して計算したin vivo蛍光値と現存量は測定値とよく合っていた。

概要 （英文）

Microalgal biofuel production has drawn much attention as biomass energy resources because of its higher potential. Among the culturable microalgal species, Botryococcus braunii is one of the promising species for the biodiesel production, because B. braunii can produce higher content of hydrocarbon than other species. Solar radiation is useful light source in mass culture system, however, the irradiance and temperature fluctuate with weather conditions, diurnally and seasonally. Fluctuation of light intensity and temperature conditions in mass culture system is known to affect productivity of microalgal biomass. To estimate biomass of B. braunii strain Showa under mass culture condition, we developed a growth model for the strain. Parameters were calculated from regressions among light intensity (0 - 2,000 micro mol photons m^-2 s^-1), temperature (15 - 38 C), incubation period, and in vivo chlorophyll-a fluorescence - biomass relationship of the strain in our previous study. Verification experiments revealed that the model well represented the changes in chlorophyll-a fluorescence and biomass of B. braunii cultured under fluctuating light and temperature conditions.

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